Anti-malonyllysine Antibody, Recombinant Antibody, R1
產(chǎn)品編號：RPTMD1M1-R1
￥ 詢價
規(guī)格 100ug
濃度 1.3mg/ml
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說明書 |
產(chǎn)品名稱：Anti-malonyllysine Antibody, Recombinant Antibody, R1
克隆號：R1
抗體亞型：Mouse IgG2b
經(jīng)驗證的應(yīng)用：WB
交叉反應(yīng)：All
特異性：SUMO-1 Antibody detects recombinant SUMO-1 and endogenous levels of sumoylated proteins.
免疫原：Synthetic peptide
宿主：CHOS/293F
來源：Recombinant Mouse IgG
純化：Protein A purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
狀態(tài)：Liquid
應(yīng)用：The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB: 0.5~5ug/ml
使用&儲存條件：Shipped at 4℃.Store at 4℃ for frequent use.Aliquot and store at -20℃ for 12 months.Avoid repeated freezing/thawing and violent shaking.Please centrifuge it, before using.
質(zhì)量驗證圖：
Figure1.Application in WB
Western blot analysis of various cell lines and tissue lysats, using RPTMD1M1-R1 at 1ug/ml dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:5000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit .
Figure2.Application in ICC
Immunofluorescent analysis of Hela cells , using RPTMD1M1-R1 (green) at 10ug/ml dilution. Nuclear DNA was labelled in blue with DAPI (blue).
別稱：Kmal, Malonyl-Lysine, 丙二?；?br />
背景信息：Lysine is subject to a wide array of regulatory post-translational modifications due to its positively charged ε-amino group side chain. The most prevalent of these are ubiquitination and acetylation, which are highly conserved among prokaryotes and eukaryotes. Acyl group transfer from the metabolic intermediates acetyl-, succinyl-, malonyl-, glutaryl-, butyryl-, propionyl-, and crotonyl-CoA all neutralize lysine’s positive charge and confer structural alterations affecting substrate protein function. Lysine acetylation is catalyzed by histone acetyltransferases, HATs, using acetyl-CoA as a cofactor. Deacylation is mediated by histone deacetylases, HDACs 1-11, and NAD-dependent Sirtuins 1-7. Some sirtuins have little to no deacetylase activity, suggesting that they are better suited for other acyl lysine substrates.SIRT5 is a predominantly mitochondrial desuccinylase and demalonylase. In the absence of a known malonyltransferase, nonenzymatic protein malonylation is likely driven by the concentration of malonyl-CoA and intracellular pH and is subject to metabolic fluctuations. Malonylation is especially prevalent among mitochondrial metabolic proteins. In type II diabetes mouse models, notably elevated malonylation can be detected mainly, but not exclusively, on proteins of glucose and fatty acid metabolism. Yeast histone H3K56 malonylation suggests poor DNA binding efficiency and may lead to reduced cell viability.
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