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Anti-KRT16 Antibody, Mouse Monoclonal, F6T

產(chǎn)品編號:MA01103HuM10-F6T
   
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規(guī)格     100ul

說明書
 

產(chǎn)品名稱Anti-KRT16 Antibody, Mouse Monoclonal, F6T
 
克隆號F6T
 
抗體亞型:/
 
經(jīng)驗(yàn)證的應(yīng)用/
 
交叉反應(yīng)Predict reacts with: Human/Mouse/Rat
 
特異性:/

免疫原A synthetic peptide of human KRT16
 
制備方法Produced in mouse immunized with KRT16, and purified by antigen affinity chromatography.
 
來源Monoclonal Mouse IgG
 
純化Immunogen affinity purified
 
緩沖液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶聯(lián)物Unconjugated
 
濃度Liquid
 
運(yùn)輸方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
儲存條件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

圖片:






Figure1.Western blot analysis of extracts of various lane1 A549、lane2 HaCAT、lane3 mouse skin, using KRT16 antibody (MA01103HuM10-F6T) at 1ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 20sec.












 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung、Monkey epididymis、Monkey esophagus、Monkey jejunum、Monkey kidney、Monkey liver、Monkey prostate、Monkey rectum、Monkey salivary gland、Monkey submandibular gland sections labelling KRT16 with purified MA01103HuM10-F6T at 5ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.

 

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