產(chǎn)品名稱:Anti-KRT17 Antibody, Mouse Monoclonal, A1T
克隆號:A1T
抗體亞型:/
經(jīng)驗證的應(yīng)用:/
交叉反應(yīng):Predict reacts with: Human/Mouse/Rat
特異性:/
免疫原:A synthetic peptide of human KRT17
制備方法:Produced in mouse immunized with KRT17, and purified by antigen affinity chromatography.
來源:Monoclonal Mouse IgG
純化:Immunogen affinity purified
緩沖液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物:Unconjugated
濃度:Liquid
運輸方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片:
Figure1.Western blot analysis of extracts of various lane1 Hela、lane2 MCF7、lane3 A431, using KRT17 antibody (MA00460HuM10-A1T) at 1ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5min.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey submandibular gland sections labelling KRT17 with purified MA00460HuM10-A1T at 5ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.
別稱:PC2, KRT17, K17, PC, PCHC1, Keratin 17, Keratin, type I cytoskeletal 17
背景信息:Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma. This antibody is specifically against KRT17.
全稱:Keratin, type I cytoskeletal 17 (KRT17)