Anti-S100A4 Antibody, Rabbit Polyclonal
產(chǎn)品編號(hào)：PA00196HuA10
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產(chǎn)品名稱：Anti-S100A4 Antibody, Rabbit Polyclonal
經(jīng)驗(yàn)證的應(yīng)用：WB/ICC
交叉反應(yīng)：Hu/Mu/Ra
特異性：human S100A4
免疫原：Recombinant human S100A4 protein, fragment Ala2~Lys101; UniprotKB: P26447
制備方法：Produced in rabbits immunized with human S100A4, and purified by antigen affinity chromatography.
來源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Western blot analysis of extracts of various cell lines and tissue, using S100A4 antibody (PA00196HuA10) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical cancer sections labelling S100A4 with purified PA00196HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution (EDTA, pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
別稱：S100-A4, MTS1, 18A2, 42A, CAPL, P9KA, PEL98, Calcium protein, Calvasculin, Metastasin, Protein Mts1, Placental calcium-binding protein, Murine placental homolog
背景信息：S100A4. S100A4 (also named metastasin, Mtsl and calvasculin) is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of proteins (1 - 5). The S100 family is a further classified as a member of the EF-hand superfamily of Ca++-binding proteins. These participate in both calcium-dependent and calcium-independent protein-protein interactions. The hallmark of this superfamily is the EF-hand motif that consists of a Ca++-binding site flanked by two alpha -helices (helix E and helix F) that were originally identified in a right-handed model of carp muscle calcium-binding protein (6). Mouse S100A4 is 101 amino acids (aa) in length (1, 2). It contains two EF hand domains, one between aa 12 - 47, and a second between aa 50 - 85. The first domain has a 14 aa cation-binding motif and binds Ca++ with low affinity. The second Ca++-binding motif is 12 aa in length and binds Ca++ with high affinity. S100A4 has no classical signal sequence but is secreted from cells (3, 7). Mouse S100A4 shares 93%, 96% and 89% aa identity with human, rat and canine S100A4, respectively. S100A4 exists as dimer (8, 9, 10). Extracellular S100A4 is reported to induce MMP production, activate MMPs, promote neurite outgrowth and stimulate cardiomyocyte proliferation (4, 10, 11, 12, 13). Within the cell, dimers are likely the functional unit. Here, they are constitutive homo- or heterodimers (with S100A1) that interact with Ca++, undergo a conformational change, and subsequently bind to cytoplasmic targets. Known targets include p53, myosin heavy chain II, F-actin and liprin beta 1 (4, 14). In general, it can be said that S100A4 blocks target phosphorylation and multimerization (4, 7, 14). S100A4 activity has been associated with cell transformation. It seems likely this is either coincidental, or a consequence, rather than a cause of transformation (3).
全稱：Protein S100-A4 (S100A4)
說明書：待上傳
經(jīng)驗(yàn)證的應(yīng)用：WB/ICC
交叉反應(yīng)：Hu/Mu/Ra
特異性：human S100A4
免疫原：Recombinant human S100A4 protein, fragment Ala2~Lys101; UniprotKB: P26447
制備方法：Produced in rabbits immunized with human S100A4, and purified by antigen affinity chromatography.
來源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Western blot analysis of extracts of various cell lines and tissue, using S100A4 antibody (PA00196HuA10) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical cancer sections labelling S100A4 with purified PA00196HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution (EDTA, pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
別稱：S100-A4, MTS1, 18A2, 42A, CAPL, P9KA, PEL98, Calcium protein, Calvasculin, Metastasin, Protein Mts1, Placental calcium-binding protein, Murine placental homolog
背景信息：S100A4. S100A4 (also named metastasin, Mtsl and calvasculin) is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of proteins (1 - 5). The S100 family is a further classified as a member of the EF-hand superfamily of Ca++-binding proteins. These participate in both calcium-dependent and calcium-independent protein-protein interactions. The hallmark of this superfamily is the EF-hand motif that consists of a Ca++-binding site flanked by two alpha -helices (helix E and helix F) that were originally identified in a right-handed model of carp muscle calcium-binding protein (6). Mouse S100A4 is 101 amino acids (aa) in length (1, 2). It contains two EF hand domains, one between aa 12 - 47, and a second between aa 50 - 85. The first domain has a 14 aa cation-binding motif and binds Ca++ with low affinity. The second Ca++-binding motif is 12 aa in length and binds Ca++ with high affinity. S100A4 has no classical signal sequence but is secreted from cells (3, 7). Mouse S100A4 shares 93%, 96% and 89% aa identity with human, rat and canine S100A4, respectively. S100A4 exists as dimer (8, 9, 10). Extracellular S100A4 is reported to induce MMP production, activate MMPs, promote neurite outgrowth and stimulate cardiomyocyte proliferation (4, 10, 11, 12, 13). Within the cell, dimers are likely the functional unit. Here, they are constitutive homo- or heterodimers (with S100A1) that interact with Ca++, undergo a conformational change, and subsequently bind to cytoplasmic targets. Known targets include p53, myosin heavy chain II, F-actin and liprin beta 1 (4, 14). In general, it can be said that S100A4 blocks target phosphorylation and multimerization (4, 7, 14). S100A4 activity has been associated with cell transformation. It seems likely this is either coincidental, or a consequence, rather than a cause of transformation (3).
全稱：Protein S100-A4 (S100A4)
說明書：待上傳