Anti-TLR4 Antibody, Rabbit Polyclonal
產(chǎn)品編號：PA00053HuA20
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產(chǎn)品名稱：Anti-TLR4 Antibody, Rabbit Polyclonal
經(jīng)驗(yàn)證的應(yīng)用：/
交叉反應(yīng)：/
特異性：human TLR4
免疫原：A synthetic peptide of human TLR4; UniprotKB: O00206
制備方法：Produced in rabbits immunized with human TLR4, and purified by antigen affinity chromatography.
來源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate sections labelling TLR4 with purified PA00053HuA20 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
別稱：CD284, TOLL, HToll
背景信息：TLR4. TLR4 is a 100 kDa type I transmembrane glycoprotein that belongs to the mammalian Toll?Like Receptor family of pathogen pattern recognition molecules. The complex of TLR4 with MD?2 functions as a critical receptor for bacterial endotoxin/lipopolysaccharide (LPS) (1-3). Mature human TLR4 consists of a 608 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 187 aa cytoplasmic domain. TLR4 contains 21 leucine rich repeats in its ECD and one cytoplasmic Toll/IL?1 receptor (TIR) domain (4). The ECD of human TLR4 shares approximately 25% aa sequence identity with other human TLRs and 60%-74% aa sequence identity with bovine, equine, feline, mouse, rat, and porcine TLR4. On monocytes, macrophages, dendritic cells, and B cells, MD-2 expression is required for cell surface localization of TLR4 and for optimal LPS-induced TLR4 signaling (5-8). MD-2 also forms soluble disulfide?linked homo?oligomers which can interact with TLR4 (6). Through a domain separate from its TLR4-binding domain, MD-2 extracts LPS from circulating CD14-LPS complexes and carries the LPS into a ternary complex with TLR4 (9-11). The interaction of MD-2/LPS with TLR4 induces receptor oligomerization and the triggering of an inflammatory response (2, 12). Increased levels of plasma MD-2 in septic shock patients sensitizes MD-2 non-expressing epithelial cells to LPS and promotes widespread tissue inflammation (13).
全稱：Toll-like receptor 4 (TLR4)
說明書：待上傳