產(chǎn)品名稱：Anti-TNF Antibody, Rabbit Polyclonal
經(jīng)驗證的應(yīng)用：WB/IHC/ICC
交叉反應(yīng)：Hu/Mu
特異性：human TNF
免疫原：Recombinant human TNF protein, fragment Gly57~L233; UniprotKB: P01375
制備方法：Produced in rabbits immunized with human TNF, and purified by antigen affinity chromatography.
來源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Western blot analysis of extracts of various tissues , using TNF antibody (PA00069HuA10) at 2ug/ml dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Blocking buffer: 5% nonfat dry milk in TBST.
Figure2.Immunofluorescent analysis of RAW 264.7 (Murine monocyte macrophages) cells.Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100,untreated or treated with 100 ng/ml lipopolysaccharides (LPS) for 7 hours labeling TNF alpha with PA00069HuA10 at 5 ug/ml.Goat anti-mouse IgG (Alexa Fluor® 488, GB25301) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution.Cytoplasmic staining was increased on RAW 264.7 cells when treated with 100 ng/ml lipopolysaccharides (LPS) for 7 hours.DAPI (blue) was used as nuclear counterstain.
別稱：DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2
背景信息：TNF-alpha. Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Bovine TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 178 aa extracellular domain (ECD) (3). Within the ECD, bovine TNF-alpha shares 64%-83% sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
全稱：Tumor necrosis factor (TNF)