產(chǎn)品名稱：Anti-FLAG Antibody, Mouse Monoclonal, F1F
克隆號：F1F
抗體亞型：/
經(jīng)驗證的應(yīng)用：WB
交叉反應(yīng)：Predict reacts with: Human/Mouse/Rat
特異性：Flag-tag
免疫原：Synthetic Peptide
制備方法：Produced in mouse immunized with FLAG, and purified by antigen affinity chromatography.
來源：Monoclonal Mouse IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
濃度：Liquid
運輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Application in WB
Western blot analysis of extracts of various lane1 10ng recombinant proteins containing the Flag Tag、lane2 50ng recombinant proteins containing the Flag Tag, using FLAG antibody (TM00975GeM20-F1F) at 1μg/mL. Secondary antibody: Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:4000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1.5min.
背景信息：/
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